Purpose : To develop a simple cheap, fast, accurate, sensitive and precise colorimetric method that can be osed for determination of chloramphenicol.
Method : Chloramphenocol was reduced in a mixture of glacial acetic acid and water using titanium (III) Chloride at room temperature within 10 min. The reduced product was then heated for 20 min with p – dimethylaminobenzaldehyde to yield the final product whose absorbance was used for the determination of the concentration of chloramphenicol. Results obtained with this method were compared with those obtained with the micribiological assay of chloramphenicol.
Result: The final product of the two step reaction was greenish – yellow in colour, absorbed strongly in the visible region and obeyed Beer’s aw at ᵞmax = 440 nm. The method developed was accurately determined chloramphenicol in the presence of common excipients and and in different dosage forms. There was statistically no significant difference (p < style="font-weight:bold;">Conclusion: A simple, fast, cheap, precise, sensitive and accurately colorimetric method has been developed that could be routinely used for the determination of chloramphenicol in bulk drug and in different dosage forms. The advantage of method is its speed and simplicity.
The original journal of An alternative colorimetric method for the determination of chloramphenocol
