Abstract
This paper described a gas chromatography – mass spectrometry (GC – MS) method for determination of alprazolam in rabbit plasma, Alprazolam and internal standart (IS) medazepam were extracted from plasma by using liquid – liquid extraction method. The samples sewe separated by GC on a DB – 5MS analytical column and determined by a quadrupole mass spectrometer detector perated under selected ion monitoring mode (SIM). Excellent linearity was found between 50 and 1000 ng/ml (r = 0.998) for plasma samples. Intra – day and inter – day precision expressed as the relative standard deviation (RSD) for the method were 1.07 – 2.69 % and 2.42 – 3.98 %, respectively. The mean recovery of alprazolam samples was 98.82 %. The limits of detection and quantification of alprazolam were 15 and 50 ng/mL, respectively. Also, the method was successfully applied to three New Zeland white rabbits which had been given an oral tablet of 1.0 mg alprazolam.
To see the original journal of Determination of Alprazolam in Rabbit Plasma by GC – MS Method
